【摘要】 参考G enB ank发表的传染性支气管炎病毒(in fectious bronch itis v irus,IBV)S基因序列,设计合成了1对引物,模拟PCR表明,该引物能扩增现已发表的所有IBV的S2基因部分核酸序列,扩增片段493 bp。建立的RT-PCR检测体系检测灵敏度达10~50 ELD50,检出限量为0.1 ng。采集上海地区12个鸡场疑似IBV禽样品34份进行检测,阳性为32份,序列分析表明均为肾变型IBV S2基因片段,S2基因同源性较高(≥99%),与其他地区肾变型IBV也有较高同源性,而与腺胃型和呼吸型相比较,序列差异较大。进化分析表明,尽管IBV S2片段变异率明显低于S1基因,但二者表现相同的进化趋势,S2基因亦可反映出IBV的分子变异及毒株间的亲缘关系。更多还原

【Abstract】 A pair of specific primer was designed and synthesized in the conserved region of S2 gene according to the infectious bronchitis virus（IBV） S sequences at GenBank.A 493 bp amplicon was abtained from simulating PCR using software SPCR3.0.The PCR system was specific with IBV while no reaction with other avian virus tested in the study.10-50 ELD₅₀ of the viron was detected.By using this method to detect the clinical samples,which were collected from 12 chicken factories,32 out of 34 suspicious samples were positive.Further analysis showed all the IBV-positive belonged to the nephropathogenic viruses.The S2 genes of the epidemic viruses share high identities with each other,while has much more difference with the proventriculopathogenic IBV and other viruses causing respiratory symptom in China.Phylogenic analysis showed though S2 fragments have less divergence among isolates than S1 genes,it shows the same phylogenic tendency with S gene among the viruses.更多还原