【摘要】 室内测定了拮抗菌株HY96-2对番茄青枯病病原菌Nb-1的毒力。平板培养法和双层培养基法结果表明,HY96-2对Nb-1具有较好的拮抗作用,其抑制率分别为20.1%和24.7%。通过温室盆栽,确定了HY96-2发酵液及其制成的生防制剂KDLD对番茄青枯病的最佳防治方法为浸根法。番茄苗浸1×105cfu/ml的HY96-2发酵液0.5h,栽入灭菌土后浇入该HY96-2发酵液100ml,处理后30d防效仍高达69.7%。温室防治试验结果表明,采用浸根法HY96-2发酵液施用后21d对番茄青枯病防效可达77.8%。生防制剂KDLD稀释100倍施用后20d防效可达81.5%。大田防治试验结果表明,生防制剂KDLD稀释100倍后使用,在番茄整个生育期对番茄青枯病都具有很好的防效,在收获末期的防效仍可达81.8%。更多还原

【Abstract】 The inhibition of biocontrol agent Paenibacillus polymyxa HY96-2 on Ralstonia solanacearum Nb-1 was determined in vitro. Result showed that the inhibition rates were 20.1% and 24.7% by dual culture and double layer culture methods,respectively.Greenhouse trials demonstrated that soaking root was the best way to use the ferment fluid of HY96-2 and its product KDLD to control tomato bacterial wilt disease.Control efficiencies were 77.8% at 21 days after soaking root with HY96-2 ferment fluid and 81.5% at 20 days after soaking with 100 times diluted KDLD.Field tests showed that 100 times diluted KDLD prevented the disease in all growing season of tomato with control efficiency 81.8% at harvest.更多还原