【摘要】 目的探讨大鼠脑缺血后,干扰素-γ(IFN-γ)是否参与免疫损伤和IFN-γ在脑缺血中的作用。方法用线拴封闭大脑中动脉的方法制作脑缺血动物模型,采用原位杂交的方法检测缺血脑组织IFN-γmRNA表达动态的变化。结果(1)缺血脑半球和假手术的脑半球相比较,在1h时IFN-γmRNA的数量就显著增加(P<0.001),且随着脑缺血时间延长IFN-γ表达量增加,6hP<0.001,12hP<0.01,24hP<0.001,48hP<0.001,6dP<0.001。(2)IFN-γmRNA表达数量损伤面积扩大而增加;结论在脑缺血过程中IFN-γmRNA表达增加,IFN-γmRNA表达主要参与大脑损伤后期反应过程。更多还原

【Abstract】 Objective To determine whether IFN-γis involved in the immune injury process and to demonstrate the function of IFN-γafter middle cerebral artery occlusion(MCAO) in rats.Methods The middle cerebral arteries(MCA) of all experimental rats were occluded by inserting nylon monofilament in the right external carotid artery.IFN-γin ischemic brain hemispheres were examined by situ hybridization(ISH) from the 1st hour to the 6th day after middle cerebral ischemic occlusion(MCAO).The T cells producing IFN-γwere analyzed by means of in situ hybridization.Results(1) The number of IFN-γmRNA in ischemic brain hemisphere was significantly more than that in shamming operation group(P<0.001) after 1 hours of MCAO and increased correspondingly as time went by after MCAO,6h P<0.001,12 h P<0.01,24 h P<0.001,48h P<0.001,6d P<0.001.(2) IFN-γmRNA expression increased correspondingly with the size of brain injury as time went by after MCAO.Conclusion The study shows that the expression of IFN-γmRNA increased during the period of ischemic brain injury,which involved in injury reaction at the later phase of brain ischemia更多还原