【摘要】 测定不同pH(7.0,7.3,7.6,8.5,9.0)环境胁迫下栉孔扇贝(Chlamys farreri)体内酸性磷酸酶(ACP)、碱性磷酸酶(ALP)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)及胞内活性氧含量(ROIs)等5种免疫指标的变化。结果表明,在pH高于正常组(pH 8.0)时,上述5种免疫指标均随pH的增加而明显升高;在pH低于正常组时情况稍复杂,4种酶活性均呈先上升后下降的趋势。ACP在pH 7.6时酶活性上升至峰值,随后酶活性降低;ALP、SOD和CAT活性均在pH 7.3时达峰值,随后酶活性降低;而胞内活性氧含量则随pH降低持续增加。实验结果表明,pH对栉孔扇贝免疫活性有明显的刺激作用,且在一定范围内随刺激强度的增加呈现免疫活性的正调节;但在高强度pH刺激下,扇贝免疫活性呈现出负调节,这种负调节可能与高强度刺激引起的免疫机能疲劳或损伤有关。[中国水产科学,2006,13(4):650-654]更多还原

【Abstract】 This study is concerned with the changes of the activities of ACP,ALP,SOD,CAT and the content of reactive oxygen species(ROIs) of Chlamys farreri at different pH levels(7.0,7.3,7.6,8.0,8.5,9.0).The results indicated that when the pH were higher than the control(pH 8.0),the five immune factors all went up with the increase of pH;when the pH values were lower than the control,the activities of the four enzymes all increased at first and then went down with the decrease of pH.The activity of ACP reached the peak at pH 7.6 and then decreased;the activities of ALP,SOD and CAT reached the peaks at pH 7.3 and then went down;but the content of ROIs always increased with the decrease of pH.The results implies pH can stimulate the immune activity significantly,and the immune activity appeares positive accomodation with the increase of stimulation at a certain range;but when the stimulation increased,the immune activity shows negative stimulation,which maybe relate with the immune fatigue or immune damnification at high stimulation.更多还原