【摘要】 目的采用PCR克隆测序方法对贵州都匀、从江,云南大理及新疆乌什4地牛带绦虫标本进行研究,与台湾桃园牛带绦虫亚洲亚种地理株作比较,为贵州株、云南株和新疆株的鉴别提供分子生物学证据,并进一步探讨牛带绦虫亚洲亚种的分类学地位。方法取贵州都匀株(DY1)、贵州从江株(CJ1),云南大理株(DL1),新疆乌什株(XJ1)和台湾桃园株(TW1)成虫节片,分别抽提DNA,PCR扩增rDNA-ITS2区段,克隆rDNA-ITS2区段后作序列分析,构建不同地理株系统发育树。结果根据ITS2序列构建的系统发育树显示:系统发育树分为3支,XJ1占据上方1支;CJ1占据中间1支;而TW1、DY1和DL1组成下方1支。结论1.DY1、DL1与TW1属于牛带绦虫亚洲亚种,CJ1、XJ1属于传统牛带绦虫;2.rDNA-ITS2序列分析可用于牛带绦虫亚洲亚种与传统带绦虫分类学之研究。更多还原

【Abstract】 The rDNA-ITS2 sequences of Taenia saginata from Duyun and Congjiang districts of Guizhou province,Dali district of Yunnan province,Wushi district of Xinjiang autonomous area were analyzed and compared with the DNA sequence of the geographical strain of Taoyuan of Taiwan in order to provide the molecular biological evidences for the identification and taxonomic classification of T.saginata.In this study,the genomic DNAs were extracted from segaments of adult tapeworms of Taiwan Taoyuan(TW1),Yunnan Dali(DL 1),Guizhou Duyun(DY1),Guizhou Congjiang(CJ1) and Xinjiang Wushi(XJ1) respectively.The rDNA-ITS2 sequences of these strains were amplified by PCR and then cloned for sequence analysis.Meanwhile,the phylogenetic tree for different geographical strains was thereby constructed.From the analysis of the phylogenetic tree esuablished for rDNA-TTS2 sequence,it was demonstrated that there were 3 branches in the phylogenetic tree,in which the upside was occupied by XJ1 strain;the middle by CJl strain and the down-side by TWl,DY1 and DL1 strains.It is concluded that DY1,DL1 and TW1 strains belong to.T saginata asiatica,whereas CJI and XJI strains belong to the typical strain of T.saginata.Therefore,the analysis of the rDNAITS2 sequence can be used for the taxonomic studies of T.siginata asiatica and the typical strain of T.saginata.更多还原