【摘要】 目的探讨X基因对肝癌细胞表达RhoC基因的影响。方法用定向克隆的方法构建X基因的真核表达载体pcDNA 3.1-X,脂质体转染HEPG 2细胞;潮霉素选择培养稳定表达X基因的HEPG 2-X细胞;免疫组化鉴定HEPG 2-X细胞RhoC蛋白表达。结果构建的真核表达载体pcDNA 3.1-X在HEPG 2细胞中有稳定表达,HEPG 2-X细胞表达RhoC蛋白增强。结论体外条件下,X基因可促进肝癌HEPG 2细胞RhoC表达增强。这可能与肝癌的侵袭、转移有关。更多还原

【Abstract】 Objective To explore the effect of HBV X gene in the expression of RhoC gene in HEPG2 cell line.Methods The whole-length X gene was directly cloned to pcDNA3.1 vector.The recombinant vector(pcDNA3.1-X) was transfected into HEPG2 cells after selection with hygromycin,steady clones(HEPG2-X cells) were obtained.The expression of RhoC protein was analysed with immunohistochemical stain.Results The recombinant plasmid could steadily express X protein efficiently,and the expression of RhoC protein in HEPG2-X cell was enhanced. Conclusions HBV X gene can enhance the expression of RhoC protein in vitro.It may be related to invasiveness and metastasis of hepatocellular carcinoma.更多还原