【摘要】 目的探讨促血管生成素2(Ang2)、缺氧诱导因子1α(HIF-1α)及血管内皮生长因子(VEGF)与肝细胞癌血管形成的关系。方法检测5 2例肝癌组织中Ang2,HIF-1α及VEGF mRNA及蛋白的表达,对共表达的肝癌组织进行微血管计数。结果RT-PCR显示,5 2例肝癌组织中有3 8例共表达Ang2mRNA,HIF-1αmRNA和VEGF mRNA,且两两之间呈明显正相关(分别为r=0.7 8 3,P<0.0 1;r=0.4 2 7,P<0.0 5;r=0.4 3 3,P<0.0 5);免疫组化发现,5 2例肝癌组织3 6例共表达Ang2,HIF-1α和VEGF蛋白。共表达Ang2 mRNA,HIFα-mRNA和VEGF蛋白的3 8例肝癌组织中,平均微血管数[(4 5.4±8.9 0)个/HP],明显高于非共表达组[(1 3.6±3.3 0)个/HP](P<0.0 5)。结论Ang2,HIF-1α和VEGF与肝癌的新生血管形成有关;肿瘤组织缺氧可能是其始动因素。更多还原

【Abstract】 Objective To investigate the relationship between hypoxia inducible factor-1α、angiopoietin2 and vascular endothelial growth factor and angiogenesis in hepatocellular carcinoma（HCC）.Methods The（expression） of hypoxia inducible factor-1α、angiopoietin2 and vascular endothelial growth factor mRNA was（detected） in 52 HCC surgical specimens.And microvessel density（MVD） in tissue specimens of patients with coexpression of the parameters was examined.Results Of the 52 surgical specimens,36 cases had over（expression） of HIF-α、angiopoietin and VEGF protein,and coexpression of HIF-α and angiopoietin and VEGF mRNA in 38 of 52 cases.The expression of HIF-α、angiopoietin was related with the expression of VEGF（r₁= 0.783,P<0.01;r₂=0.427,P<0.05;r₃=0.433,P<0.05）.In the cases of over（expression） of HIF-α、angiopoietin and VEGF protein and mRNA,the average MVD was[（45.4 ±8.90）/HP] significantly higher than that of cases with non coexpression group[（13.6±3.30）/HP]（P<（0.05））.Conclusions HIF-α、angiopoietin and VEGF are possible angiogenic factors in the angiogenesis in HCC.Tumor tissue hypoxia may be the initiating factor.更多还原