【摘要】 目的探讨肝移植对肝硬变大鼠脾脏功能的影响。方法制备四氯化碳中毒性肝硬变大鼠模型,采用“二袖套”法进行肝移植。观察肝移植前、后大鼠脾指数及脾脏组织形态学的变化;应用免疫荧光染色技术及流式细胞仪检测肝移植前、后大鼠脾脏T淋巴细胞亚群的变化。结果肝移植前,肝硬变大鼠脾指数增高,从(2.42±0.11)mg/g增至(3.62±0.14)mg/g,P<0.01;脾脏白髓面积缩小,从(23.47±2.30)%缩至(7.70±2.01)%,P<0.01;脾小梁面积扩大,从(1.75±0.61)%增加至(4.46±0.71)%,P<0.01;脾脏T淋巴细胞亚群CD4/CD8比值明显下降,从2.67±0.15降至1.18±0.15,P<0.01。肝移植后,随着时间的延长,大鼠脾脏白髓面积有所扩大,从(7.70±2.01)%增至(15.07±1.97)%,P<0.01;脾小梁面积有所缩小,从(4.46±0.71)%降至(3.11±0.51)%,P<0.05;脾指数逐渐降低,从(3.62±0.14)mg/g降至(2.62±0.11)mg/g,P<0.01;脾脏T淋巴细胞亚群中CD4/CD8比值也有所升高,从1.18±0.15升至2.32±0.11,P<0.01。结论肝硬变大鼠行肝移植后,因肝功能严重损害而导致的脾脏功能异常可得到改善。更多还原

【Abstract】 Objective To investigate the effects of liver transplantation on splenic function in rats with hepatic cirrhosis.Methods Hepatic cirrhosis model was established in rats by subcutaneous injections of carbon tetrachloride.Liver transplantation model was established with two-cuff technique.Spleen index,morphological changes of spleen were observed before and after liver transplantation in hepatic cirrhosis rats.Spleen T lymphocyte subgroups before and after liver transplantation were also assayed by immunofluorescence staining and flow cytometry.(Results Before) liver transplantation,spleen index was increased from(2.42±0.11) mg/g to(3.62±0.14) mg/g,P<0.01;pathological examination of spleen samples showed that the areas of white pulp were decreased from((23.47±2.30))% to(7.70±2.01)%,P<0.01,and the areas of spleen trabecula were increased from(1.75±(0.61))% to(4.46±0.71)%,P<0.01.Meanwhile,the ratio of CD4/CD8 of spleen T lymphocyte subgroups was decreased from 2.67±0.15 to 1.18±0.15,P<0.01.After liver transplantation,spleen index was decreased from(3.62±0.14) mg/g to(2.62±0.11) mg/g,P<0.01;pathological examination of spleen showed that the areas of white pulp were increased from(7.70±2.01)% to(15.07±1.97)%,P<0.01,and those of spleen trabecula were decreased from(4.46±0.71)% to(3.11±0.51)%,P<0.05.Meanwhile,the ratio of CD4/CD8 of spleen T lymphocyte subgroups was increased from 1.18±0.15 to 2.32±0.11,P<0.01.Conclusion Impaired function of spleen resulting from liver function damage can be improved in rats with hepatic cirrhosis after liver transplantation.更多还原