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ECTOINE对糖化酶在逆环境下的保护作用
Protection of ectoine towards glucoamylase at stress condition
【摘要】 研究了ectoine对糖化酶在某些逆环境下的保护作用。结果表明e:ctoine对经高温、干燥、低温、高浓度盐条件处理的糖化酶均有明显的保护作用,4.0mmol/L的ectoine可使经90℃处理的糖化酶的残余酶活力提高49.2%;4.5 mmol/L的ectoine可使经干燥处理的糖化酶的残余酶活力提高49.8%;使经-20℃处理的糖化酶的残余酶活力提高21.7%;3.0mmol/L的ectoine可使经4mol/LNaCl处理的糖化酶的残余酶活力提高19.1%。
【Abstract】 The protective effect of ectoine on glucoamylase under stress conditions was studied.The results indicated that ectoine protected glucoamylase obviously against various stress,such as high temperature,dryness,low temperature,high concentration of NaCl.When concentration of ectoine was 4.0 mmol/L,residual enzyme activity of glucoamylase at 90℃ was improved 49.2%.When concentration of ectoine was 4.5 mmol/L,residual enzyme activity of glucoamylase was improved 49.8%,residual enzyme activity of glucoamylase at-20℃ was improved 21.7%.Ectoine of 3.0 mmol/L enhanced residual enzyme activity of glucoamylase 19.1% under 4 mol/L of NaCl.
【Key words】 ectoine; glucoamylase; residual enzyme activity; enzymatic protective reagent;
- 【文献出处】 中国酿造 ,China Brewing , 编辑部邮箱 ,2006年06期
- 【分类号】TS201.3
- 【被引频次】9
- 【下载频次】157