【摘要】 目的:观察清络通痹颗粒对胶原性关节炎大鼠滑膜凋亡相关基因的影响。方法:采用牛Ⅱ型胶原制备大鼠胶原性关节炎模型,提取滑膜细胞总RNA,采用RTPCR技术,检测药物对滑膜细胞p53mRNA、bcl2mRNA的影响。结果:胶原性关节炎大鼠滑膜细胞p53mRNA、bcl2mRNA表达较正常组明显升高(P<0.01),清络通痹颗粒7.2g/kg可明显下调胶原性关节炎大鼠滑膜细胞p53mRNA、bcl2mRNA的水平(P<0.05,P<0.01)。结论:清络通痹颗粒可能通过下调滑膜细胞凋亡相关基因p53mRNA、bcl2mRNA表达,促进滑膜细胞的凋亡而发挥治疗作用。更多还原

【Abstract】 Objective:To investigate the effects of apoptosis of Qingluotongbi Granule(QLT) on the expression of p53 mRNA and bcl-2 mRNA in synovium of collagen induced arthritis(CIA) rats.Methods:The typeⅡ collagen from beef was used to induce the rat model of CIA. 7.2 g/kg QLT treatment was used as experimental group. The total RNA was extracted from synoviocyte. p53 mRNA and bcl-2 mRNA were analyzed by RT-PCR.Results:The expression of p53 and bcl-2 mRNA was higher in the synoviocyte of CIA rats than that in normal control(P<0.01). The expression of p53 and bcl-2 mRNA in synoviocyte of CIA rats were decreased significantly after QLT treatment(P<0.05 and P<0.01, respectively).Conclusion:QLT might have the function on decreasing the expression of p53 and bcl-2 mRNA and promote the apoptosis of synoviocyte.更多还原