【摘要】 目的探讨凋亡抑制基因(IAPs)家族在非小细胞肺癌(NSCLC)患者组织中的表达及其意义。方法采用半定量反转录聚合酶链反应(RT-PCR)及Western blot分别从mRNA和蛋白水平对36例NSCLC、36例肺部良性病变进行研究,检测生存蛋白(survivin),human IAP-1(hIAP-1),humanIAP-2(hIAP-2)及X chromosome-linked IAP(XIAP)表达情况。结果36例NSCLC组织中有32例survivin mRNA表达阳性,而对照组无一例阳性表达;26例癌组织中XIAP mRNA处于高表达,与对照组比较差异有统计学意义(P<0.05);hIAP-1mRNA仅仅在腺癌组织中高表达(P<0.05),具有组织学类型特异性。Western blot显示在survivin mRNA阳性组织中均可检测到survivin蛋白的表达;XIAP和hIAP-1mRNA高表达的组织同样可检测到相应蛋白。相关性分析表明只有hIAP-1的表达与NSCLC组织学类型相关。结论survivin mRNA高表达可以作为NSCLC辅助诊断的重要指标,hIAP-1可用来协助鉴定NSCLC亚型,survivin和XIAP在肿瘤基因治疗中具有潜在的应用价值和前景。更多还原

【Abstract】 Objective To investigate the expression of inhibitors of apoptosis (IAPs) in non-small cell lung cancer (NSCLC) patients and its clinical significance. Methods The mRNA expression level and protein level of survivin, hIAP-1 (human IAP-1), hIAP-2 (human IAP-2), and XIAP (X chromosome-linked IAP) in 36 NSCLC patients and 36 controls were analyzed by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blotting, respectively. Results High survivin mRNA expression were detected in 32 out of 36 patients, but not in controls. 26/36 NSCLC showed high XIAP mRNA expression and were significantly higher than those with benign diseases (P < 0.05). An elevated hIAP-1 mRNA expression was detected especially in patients with adenocarcinoma (P < 0.05). The tumor specimens with high survivin mRNA expression were also characterized by an elevation in the respective protein level. The enhanced mRNA expression of hIAP-1 and XIAP in respective tumors could also be confirmed on protein level by Western blotting. There was a significant correlation between the expression of hIAP-1 and histopathological type. Conclusion High survivin mRNA expression could be a valuable parameter in NSCLC diagnosis. hIAP-1 might be used to distinguish the subtype of NSCLC. Survivin and XIAP have potentially applied prospect in tumor gene therapy.更多还原