【摘要】 目的:观察匹格列酮对链脲佐菌素(STZ)诱导的糖尿病大鼠肿瘤坏死因子-α(TNF-α)表达的影响。方法:将SD大鼠随机分为正常对照组、糖尿病组和匹格列酮治疗组,匹格列酮灌胃8周时处死大鼠,腹主动脉取血,分别检测各组血糖、糖化血红蛋白、尿微量白蛋白以及血浆TNF-α水平,取大鼠肾皮质提取RNA,用RT-PCR方法检测TNF-αmRNA表达的变化。结果:8周时糖尿病组大鼠血糖、糖化血红蛋白、血肌酐、尿素氮、尿微量白蛋白以及血浆TNF-α水平均显著高于正常对照组,用匹格列酮治疗后上述指标均较糖尿病组显著下降,TNF-αmRNA表达也显著降低。结论:匹格列酮能抑制TNF-α浓度的升高,下调TNF-αmRNA的表达,提示匹格列酮对糖尿病大鼠肾脏具有一定的保护作用。更多还原

【Abstract】 Objective:To investigate the effects of Pioglitazone on the expression of tumor necrosis factor-α(TNF-α)in the renal cortex of diabetic rats.Methods: SD rats were divided into three groups randomly: normal control group,diabetic group,and diabetic group with Pioglitazone treatment.At 8th week,blood glucose,HbA1c,u-Albumin,serum creatinine,urea nitrogen and TNF-αlevel in blood plasm were detected in three groups.the expression of TNF-αmRNA was detected in renal cortex by RT-PCR. Results: Blood glucose,glycosylated hemoglobin,urine micro albumin,serum creatinine,urea nitrogen and TNF-αlevel in blood plasm and the expression of TNF-αm RNA were all increased significantly in dialetic group compared with contol group,but they were much lower in diabetic group with Pioglitazone treatment. Conclusion: Pioglitazone could obviously reduce the expression of TNF-α in renal cortex of diabetic rats and it may protect the renal.更多还原