【摘要】 目的:测定不同浓度中药柴胡(Bup leurun Ch inese DC,BCDC)提取物对BEL-7402细胞内游离钙离子浓度([Ca2+]i)和p53表达的影响,以探索柴胡逆转BEL-7402细胞多药耐药的机制。方法:用Fura-2/AM作为细胞内钙离子的荧光指示剂,用双波长荧光分光光度计测定不同浓度柴胡提取物作用下BEL-7402细胞内[Ca2+]i及其剂量-效应关系;用免疫组化法检测不同条件下BEL-7402细胞的p53表达。结果:柴胡可使人肝癌细胞BEL-7402细胞内游离钙离子浓度下降(P<0.001),但不具有剂量-效应关系;BEL-7402细胞呈p53天然突变。结论:柴胡可使人肝癌细胞BEL-7402细胞内游离钙离子浓度下降,为一种钙离子通道阻滞剂(calc ium channel b locker,CCB),提示钙离子通道阻滞作用为柴胡逆转BEL-7402细胞多药耐药的机制之一;BEL-7402细胞呈p53天然突变也可直接或间接影响肿瘤多药耐药性的表达。更多还原

【Abstract】 Objective: To measure the effects of the extracts from BCDC on intracellular free calcium and the expression of p53of human hepatoma BEL-7402 cells,in order to explore the mechanism of BCDC reversing the multidrug resistance of BEL-7402 cells of human hepatoma.Methods: Double wavelength spectrofluorometer with Fura-2/AM as the fluorescence indicator for intracellular free calcium ions([Ca²⁺]i) was used to measure the changes of [Ca²⁺]i in BEL-7402 cells of human hepatoma affected by BCDC and the expression of p53 was examined by immunochemical assay.Results: BCDC can reduce intracellular free calcium of human hepatoma BEL-7402 cells（P<0.001）.Immunochemical assay showed that BCDC had no effect on p53 gene expression.Conclusion: BCDC is one of calcium channel blocker and is able to reduce intracellular free calcium of human hepatoma BEL-7402 cells,which indicats that the effect of calcium channel blocker may be one of the mechanism of BCDC reversing the mutidrug resistance of BEL-7402 cells of human hepatoma.And the p53 mutation of BEL-7402 cells can affect the expression of MDR1.更多还原