【摘要】 目的观察心肺复苏大鼠心肌组织超微结构变化以及乌司他丁(Ulinastatin,UTI)的影响,探讨心肺复苏大鼠心肌损伤的机制及乌司他丁的治疗机制。方法利用窒息导致大鼠心脏骤停后心肺复苏的动物模型,将Wistar大鼠随机分为3组：对照组(假手术组)、常规复苏组、乌司他丁组,每组8只。于自主循环恢复(ROSC)2h取动脉血,采用酶生化法测定血清中CK-MB的含量；放射免疫法测定血清中TNF-α的含量,采用透射电镜(TEM)观察心肌细胞的损伤情况。结果与常规复苏组比较,乌司他丁组血清中TNF-α的含量显著降低,心功能改善,心肌组织病变减轻。结论大鼠心肺复苏后,血清TNF-α升高参与心肌损伤过程,乌司他丁对复苏后早期的心肌损伤有保护作用。更多还原

【Abstract】 Objective To study the changes of myocardial uhrastructure and effects of Ulinastatin(UTI)on rats after cardiop- ulmonary resuscitation(CPR),and to investigate their mechanism.Methods Wistar rats were randomly divided into three groups: sham control group;routine treatment group;UTI treatment group(n=8,per group).Cardiac arrest was induced by asphyxiation in groups except A and CPR was performed.Two hours after recovery,arterial blood was drew for determination of serum TNF-αand CK-MB.The ultrastructural damages were observed with transmission electron microscope(TEM).Results Compared with routine treatment group,serum level of TNF-αin UTI treatment group decreased significantly and heart function improved.The damage of myocardial ultrastructure was ameliorated markedly.Conclusions TNF-αis probably the extremely important factors that promote the myocardial injury.Ulinastatin can improve the heart function of rats during early stage of resuscitation,and may alleviate the myo- cardial injury.更多还原