【摘要】 目的探讨多肿瘤抑制基因1(MTS1)和环氧合酶-2(COX-2)基因在浸润性乳腺癌组织中的表达及其临床意义。方法应用流式细胞术(FCM)定量分析66例原发性浸润性乳腺癌患者癌组织、相应癌旁组织中MTS1和COX-2的表达。结果浸润性乳腺癌组织中MTS1和COX-2蛋白表达的相对荧光强度(RFI)中位数分别为0．84和10．54,而相应癌旁组织分别为1．61和4．00,癌组织MTS1和COX-2蛋白表达与相应癌旁组织比较,差异有统计学意义(P<0．05)。MTS1和COX-2在不同年龄、病理类型、肿瘤大小和临床分期间的表达差异均无统计学意义(P>0．05)。有淋巴结转移的患者MTS1和COX-2表达中位数分别为1．12和5．94,无淋巴结转移患者分别为0．79和13．05,差异均有统计学意义(P<0．05)。结论MTS1和COX-2在乳腺癌的发生、发展中起重要作用,并与淋巴结转移状况有关,可作为预测乳腺癌预后的指标。更多还原

【Abstract】 Objective The purpose of the present study was to explore the expression and clinical significance of multiple tumor suppressor gene 1 ( MTS1) and cyclooxygenase-2 ( COX-2) gene in invasive breast cancers. Methods Flow cytometry was used to analyze the expression level of MTS1 and COX-2 in cancer tissues and corresponding para-cancer tissues from 66 cases of primary invasive breast cancers. Results In breast cancer tissues, the expression of MTS1 and COX-2 assessed by relative fluorescence intensity were 0. 84 and 10. 54, respectively, and were 1. 61 and 4. 00 in corresponding para-cancer tissues, respectively. There was a significant difference between MTS1 and COX-2 expressions in cancer and corresponding para-cancer tissues (P <0. 05) . The differences of MTS1 and COX-2 expression of different ages , pathological types, tumor sizes or clinical stages of the breast cancer patients were not significant ( P > 0.05). The MTS1 and COX-2 expressions were 1. 12 and 5.94, respectively, in lymph node metastasis positive patients, and 0. 79 and 13. 05, respectively, in lymph node metastasis negative patients. The differences were significant (P <0. 05) . Conclusion The preliminary research results suggest that MTS1 and COX-2 gene expressions play fairly important role in tumorigenesis and progression of breast cancers. MTS1 and COX-2 protein expressions have correlation with lymph node metastasis. This study provides theoretical basis for use of COX-2 selective inhibitors in prevention and treatment for breast cancer patients.更多还原