【摘要】 目的探讨Y染色体非重组区HindⅢ酶切位点多态性与中国汉族人群原发性高血压的关系。方法入选男性研究对象857例,其中原发性高血压患者402例、正常对照人群455名。所有研究对象用常规方法提取白细胞DNA,采用多聚酶链反应结合限制性内切酶(HindⅢ)方法检测Y染色体非重组区HindⅢ酶切位点多态性。结果对照组和原发性高血压组Y染色体HindⅢ酶切位点多态性比较各基因型差异有统计学意义(P=0·02),高血压患者HindⅢ(+)者基因型为58·5%(235/402),正常人群为66·4%(302/455);高血压患者HindⅢ(+)者基因型少于正常人群,HindⅢ(-)者基因型增多。HindⅢ(+)者基因型收缩压(133·8mm Hg±25·2mm Hg),舒张压(83·5mmHg±13·3mm Hg)和平均动脉压(100·2mm Hg±16·5mm Hg)均明显低于HindⅢ(-)者基因型(分别为138·0mm Hg±27·0mm Hg、85·9mm Hg±14·4mm Hg、103·3mm Hg±17·6mm Hg,均P<0·05)。结论Y染色体HindⅢ酶切位点多态性与原发性高血压有关,可能是中国汉族人群原发性高血压的一个遗传标志。更多还原

【Abstract】 Objective To examine whether there is an association essential hypertension pressure and a polymorphic HindⅢ biallelic marker in the non-recombining region of Y chromosome in Chinese Han people. Methods Peripheral blood samples were collected from 402 males with essential hypertension pressure and 455 age- and body height-matched healthy males as control group. Genomic DNA was extracted from the white blood cells. Segments of polymorphic HindⅢ restriction site of the Y chromosome were amplified from the genomic DNA by polymerase chain reaction(PCR). The PCR products were restricted with 10 U of HindⅢ overnight at 37℃.The digested products were subjected to electrophoresis in 3% agarose gels, and stained with ethidium bromide. Results The HindⅢ(+)genotype was found in 58.5% of the men with essential hypertension(235/402), significantly lower than that in the healthy men (64.4%, 302/455, P=0.02). The systolic blood pressure of the men with HindⅢ(+)genotype was 133.8 mm Hg±25.2 mm Hg, significantly lower than that of the HindⅢ(-) genotype (138.0 mm Hg±27.0 mm Hg,P<0.05), and the diastolic blood pressure (DBP) of the men with HindⅢ(+)genotype was 83.5 mm Hg±13.3 mm Hg, significantly lower than that of the men with HindⅢ(-) genotype (85.9 mm Hg±14.4 mm Hg, P=0.01), and the mean arterial pressure of the men with HindⅢ(+)genotype was 100.2 mm Hg ±16.5 mm Hg, significantly lower than that of the of the men with HindⅢ(+)genotype was (103.3 mm Hg±17.6 mm Hg, P=0.01). Conclusion Polymorphic HindⅢ restriction site of the Y chromosome is associated with essential hypertension in Chinese Han people.更多还原