【摘要】 目的探讨口服中药熊胆粉透过血-眼屏障到达相应靶组织的机制及其作用。方法56只家兔按照随机数字表法分成对照组8只和给药组48只。给药组再随机分成6个亚组,每个亚组8只,空腹12h给予兔中药熊胆粉100mg/kg体重灌胃,分别在灌胃后0.5、1.0、2.0、4.0、6.0、8.0h于家兔耳缘静脉采血2ml,均左眼取房水、右眼取玻璃体。对照组的实验方法同给药组,处理时间记为0h。利用高效液相色谱仪法（HPLC）测定熊胆粉中标记物牛磺熊脱氧胆酸（TUDCA）在血液、房水、玻璃体内的浓度。结果利用HPLC可准确测血液、房水、玻璃体内TUDCA浓度,其中血液中浓度为（999.1±17.2）^1300.6±78.2μg/ml;房水中浓度为（12.7±1.4）^47.8±4.7μg/ml;玻璃体中浓度为（10.8±2.9）^57.9±7.9μg/ml。给药组各时间点血液、房水、玻璃体中TUDCA的浓度分别与对照组相比,差异有统计学意义（P<0.01）;不同时间点房水和玻璃体内TUDCA浓度之间的比较,差异无统计学意义（P>0.05）。结论口服中药熊胆粉能透过血-眼屏障到达相应靶组织,于房水和玻璃体中的浓度明显小于血液中浓度,进入房水和玻璃体的能力相近。（中华眼科杂志,2006,421023-1025）更多还原

【Abstract】 Objective To determine whether Tauroursodeoxycholic acid (TUDCA) can penetrate the blood-ocular barrier after orally administration of Fel Ursi. Methods 56 rabbits were divided into two groups, 48 rabbits were used in experimental group, and other 8 rabbits were served as control. 100 mg/ml Fel Ursi were affused into rabbits stomach. 2 ml blood from vein of auris-edge, aqueous humor from left eye and vitreous sample from right eye were obtained at 0.5,1.0,2.0,4.0,6.0 and 8.0 h after Fel Ursi administration. Concentration of TUDCA from all of samples was determined by HPLC. Results TUDCA Concentrations were (999.1±17.2)-(1300.6±78.2)μg/ml, (12.7±1.4)-(47.8 ±4.7)μg/ml, and (10.8±2.9)-(57.9±7.9)μg/ml in blood, aqueous humor and vitreous respectively . There was no significant differences in the concentration of TUDCA in samples of aqueous humor and vitreous (P>0.05). However the concentration of TUDCA in rabbit blood was much higher compared with that in aqueous humor and vitreous (P<0.01).Conclusion Fel Ursi can reach intraocular tissue through penetrating blood-aqueous barrier and blood-vitreous barrier after orally application.更多还原