【摘要】 目的探讨亚低温对幼猪外伤性脑损伤(traum atic brain in jury,TB I)的保护作用。方法将幼猪TB I模型(液压法)随机分为常温对照组(颅温37.5℃~38.5℃;n=10)和亚低温组(34℃±0.2℃;n=10)。检测脑脊液中特异性烯醇化酶(NSE)、S-100蛋白(S-100)、磷酸肌酸激酶脑型同工酶(CK-BB)水平变化,监测颅内压的变化以及病理检查脑梗塞体积及脑损伤积分。结果常温组TB I后48 h颅内压达高峰,峰值(38.7±6.7)mmHg,亚低温组颅内压均维持在较低水平,各时点指标均比常温对照组明显降低(P<0.01);TB I后24 h、48 h、72 h亚低温组脑脊液NSE水平较常温对照组明显降低(P<0.01);亚低温组脑脊液S-100、CK-BB测定值亦明显低于常温对照组各个时点的水平(P<0.05、P<0.001);脑损伤积分及脑梗塞体积亚低温组分别为(10.2±3.0)mm3和(38.4±23.5)mm3,显著少于常温对照组(16.4±4.2)mm3、(85.6±18.2)mm3;P<0.05。结论颅内压、脑脊液生化指标物NSE、S-100、CK-BB水平,病理分析证实亚低温对幼猪TB I具有较好的脑保护作用。更多还原

【Abstract】 Objective To investigate the protective effects of moderate hypothermia on the traumatic brain injury(TBI) in piglets.Methods The TBI model were generated by the fluid percussion method in 20 piglets,and these piglets were then randomly divided into normal temperature group(intracranial temperature(37.5℃)38℃,n=10) and moderate hypothermia group(intracranial temperature(34℃)±(0.2℃),n=10).The intracranial pressure(ICP) levels,brain injury scores,and infarct volumes were recorded in all piglets.And the levels of NSE,S-100,and CK-BB in the cerebrospinal fluid(CSF) were measured.Results The peak levels of ICP in normal temperature group were reached at 48h after TBI.The ICP levels in the moderate hypothermia group were lower than those in normal temperature group at each given time-point(P<(0.01)).And the brain injury scores and infarct volumes in the moderate hypothermia group were lower than those in the normal temperature group(P<(0.05)).The levels of NSE,S-100,CK-BB in the moderate hypothermia group were also lower than those in the normal temperature group at 24h,48h,and 72h after TBI.Conclusions Moderate hypothermia shows the protective effects on the piglets with TBI.更多还原