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重组人白介素6对SW872脂肪细胞促酰化蛋白分泌的影响
Effect of recombinant human interleukin-6 on the secretion of acylation-stimulating protein in SW872 adipocytes
【摘要】 体外培养SW872细胞,油酸诱导其分化为成熟的脂肪细胞,然后加入重组人白细胞介素6 (IL-6)观察其对SW872成熟脂肪细胞促酰化蛋白(ASP)分泌的影响。结果显示0.6mmol/L油酸刺激72 h能成功诱导SW872前脂肪细胞分化为成熟的脂肪细胞;IL-6对SW872成熟脂肪细胞ASP的分泌具有明显的抑制作用,且表现为时间和剂量依赖效应。
【Abstract】 SW872 cells were cultured in vitro with oleic acid and differentiated into mature adipocytes. The role of interleukin-6(IL-6)in the secretion of acylation-stimulating protein(ASP)in mature SW872 adipocytes was observed.The results suggested that IL-6 significantly inhibited ASP secretion into the media in a dose-and time-dependent manner in mature SW872 adipocytes.
【关键词】 SW872细胞;
脂肪细胞;
细胞分化;
油酸;
白细胞介素6;
促酰化蛋白;
【Key words】 SW872 cells; Adipocytes; Cell differentiation; Oleic acid; Interleukin-6; Acylationstimulating protein;
【Key words】 SW872 cells; Adipocytes; Cell differentiation; Oleic acid; Interleukin-6; Acylationstimulating protein;
【基金】 国家自然科学基金(No.30170442);国家自然科学基金国际合作交流项目(No.30311120163)
- 【文献出处】 中华内分泌代谢杂志 ,Chinese Journal of Endocrinology and Metabolism , 编辑部邮箱 ,2006年03期
- 【分类号】R589.2
- 【被引频次】1
- 【下载频次】63