【摘要】 运用细胞原代培养技术、愈创木酚法及同位素法,观察不同浓度硝普钠(SNP)对甲状腺细胞甲状腺过氧化物酶(TPO)及碘有机化的影响。0、10-6、10-5、10-4和10-3mol/LSNP呈剂量依赖性抑制碘有机化[分别为(2708±250,2471±186,2319±159,1464±143,596±161)cpm/孔]。SNP(10-6~10-4mol/L)短时间(0.5h)孵育可增强甲状腺细胞TPO活性(0.027±0.004,0.033±0.002,0.050±0.003,0.040±0.003)U×10-5,10-3mol/LSNP对TPO活性没有影响(0.025±0.005)U×10-5。不同浓度的SNP长时间(72h)孵育未显示对甲状腺细胞TPO活性有影响(P>0.05)。碘有机化与TPO活性不相关。以上结果提示,NO抑制甲状腺细胞碘有机化,此作用可能与TPO无关。更多还原

【Abstract】 Thyroid cells obtained from paraadenomatous tissue of thyroid adenomas were cultured in a monolayer system.The iodideorganification was assayed in thyrocytes by radioactive isotope. Change in activity of thyroid peroxidase (TPO) was assayed by guaiacol, which is related to iodide organification. 0, 10-6, 10-5, 10-4 and 10-3 mol/L nitroprusside (SNP)significantlyinhibitedtheiodideorganificationin a dose-dependent manner(2708±250, 2471±186, 2319±159, 1464±143, 596±216)cpm/well. When thyrocytes were incubated with 0 or 10-6-10-4 mol/LSNPfor0.5 h,TPOactivities were enhanced [(0.027±0.004; 0.033±0.002; 0.050±0.003; 0.040±0.003)U×10-5],butnotundertheconcentration of 10-3mol/L (0.025±0.005)U×10-5. As thyrocytes were incubated with various concentrations of SNP for 72 h, TPO activity did not show statistical change. TPO activity was not related to iodide organification. The results suggested that NO inhibits iodide organification, and this action is probably not related to the activity of TPO in thyrocytes.更多还原