【摘要】 目的探讨二硫化碳对离体培养支持细胞凋亡及FasL基因表达的影响。方法以0、0．36、0．72、1．44μmol/ml浓度的二硫化碳作用于离体培养的大鼠支持细胞24 h后,应用噻唑蓝(MTT)法检测支持细胞存活率,流式细胞术检测细胞凋亡及逆转录聚合酶链反应(RT-PCR)技术检测凋亡相关基因FasL的表达。结果二硫化碳处理后的支持细胞存活率随作用浓度的增加而下降,当≥1．44μmol/ml浓度时,细胞存活率(73．34%±1．39%)明显下降,与溶剂对照组(99．98%±5．48%)比较,差异有统计学意义(P＜0．05)。随二硫化碳浓度升高,细胞凋亡率增加,当浓度≥1．44μmol/ml时,支持细胞凋亡率(7．93%±0．43%)明显升高,与溶剂对照组(3．63%±0．53%)比较,差异有统计学意义(P＜0．05)。随二硫化碳浓度增加,凋亡相关基因FasL表达量亦明显增加。结论二硫化碳对离体培养支持细胞具有细胞毒性作用,可导致细胞凋亡率升高,凋亡相关基因FasL高表达。更多还原

【Abstract】 Objective To study apoptosis and genc FasL expression induced by carbon disulfide in steroli cells of male rats.Methods Steroli cells were exposed to different concentrations of CS2 （0,0.36,0.72,1.44μmol/ml） for 24 hours.Survival rate,apoptosis rate,expression level of gene FasL were measured using MTT, FCM,and RT-PCR methods respectively.Results Steroli cell survival rate decreased as the concentration of CS₂ increased.The survival rate（73.34%±1.39%） was significantly lower than the control group（99.98%±5.48%）when the concentration of CS₂≥1.44μmol/ml（P＜0.05）.Apoptosis rate increased as the CS₂ concen- tration increased.Apoptosis rate（7.93 %±0.43%）was significantly higher when the concentration of CS₂≥1.44μmoL/ml（P＜0.05）.Expression level of the FasL significantly increased as the concentrations of CS₂（P＜0.05）. Conclusion CS₂ is cytotoxic to steroli cells.It could cause apoptosis of steroli cells.更多还原