【摘要】 目的研究模拟微重力条件下构建新生大鼠神经细胞培养体系的方法。方法分离新生鼠海马区原代神经细胞,接种于Cytodex3型微载体,培养3 d后转移入旋转细胞培养系统,用倒置显微镜和扫描电镜观察神经细胞在微载体上的生长情况,进行神经元细胞特异性烯醇酶的免疫组织化学染色,用Image-Pro Plus软件进行神经细胞胞体面积的测量。结果微重力条件下神经细胞在微载体上均匀分布,免疫组织化学结果显示神经元被染成棕色,其胞体面积较正常重力条件培养的神经细胞有显著增加(P＜0．01)。结论神经细胞可以在微重力条件下进行培养,这个体系的建立有着广泛的应用前景。更多还原

【Abstract】 Objective To develop neuron culture systems under the condition of simulated mi- crogravity in rats. Methods Primary neurons were separated from the neonatal rats, seeded on Cy- todex3 micro-carriers, cultured for 3 days, and then moved into rotary cell culture system. The growth of neurons on micro-carriers was observed under inverted phase contrast microscope, immuno- cyto-chemical analysis against neuron specific enolase was performed in microgravity-cultured neurons, the area of cell body was measured by the software of Image-Pro Plus. Results Neurons attached to micro-carriers distributed well under the condition of simulated microgravity, and were stained posi- tively for neuron specific enolase. Compared with normal gravity, the area of cultured neurons cell body increased significantly under simulated microgravity. Conclusions It is suitable to develop neuron culture systems under the condition of stimulated microgravity and this method may be poten- tially valuable in space medicine.更多还原