【摘要】 目的研究乙型肝炎表面抗原（HBsAg）与β₂糖蛋白Ⅰ（β₂GPⅠ）的结合特性,探讨β₂GPⅠ在乙型肝炎病毒（HBV）嗜肝性中的作用。方法采用Western blot技术,研究还原型与非还原型β₂GPⅠ与HBsAg的结合特性。结果还原型与非还原型β₂GPⅠ与HBsAg有相同的亲和力,推测β₂GPⅠ与HBsAg的结合部位是在一级结构上相邻的氨基酸残基肽段,与空间构象关系不大。结论HBV与β₂GPⅠ有特异的亲和能力,这一亲和能力可能在HBV感染肝细胞过程中发挥了重要作用,是HBV嗜肝性的原因之一。更多还原

【Abstract】 Objective To further study the binding character of hepatitis B surface antigen （HBsAg） and beta 2-glycoproteinⅠ（β₂GPⅠ） and to explore whetherβ₂GPⅠplays an important role in the hepatotropism of hepatitis B virus. Methods Using Western blot technique, we observed the binding character of the HBsAg with reduced and non-reducedβ₂GPⅠ. Results rHBsAgs with reduced and non-reducedβ₂GPⅠshowed identical binding activity. Conclusions The binding activity of HBsAg is dependent on tandem residues, but not on conformational structures ofβ₂GPⅠ. There is a specific binding between HBV andβ₂GPⅠ, which may play an important role in HBV infection and is one of the reasons of hepatotropism of HBV.更多还原