【摘要】 目的探讨乙型肝炎病毒(HBV)自限感染和慢性感染与粘病毒抵抗基因-1(MxA)启动子的-88位点 G／T单核苷酸多态性的关系。方法收集100例抗-HBs和抗-HBc阳性的HBV自限感染者和340例慢性感染者的外周全血,提出基因组DNA;采用竞争分化聚合酶链反应技术为基础的方法进行MxA-88 G／T基因分型;采用单因素Odds ratio和x2检验等方法进行统计学分析。结果MxA-88 G／G基因型(低表达型)检出率为50．2％(221／440), T／T基因型(高表达型)检出率为5．5％(24／440),G／T杂合型检出率为44．3％(195／440)。与慢性感染患者相比, 自限感染患者携带较低的G／G基因型(41．0％与52．9％,P<0．05)、G等位基因(62．5％与75．3％,P<0．01)和较高的T／T基因型(16．O％与2．4％,P<0．01)、T等位基因(37．5％与24．7％,P<0．01),而两者之间的G／T 杂合型差异无统计学意义。结论MxA-88 G／T基因型能在一定程度上影响HBV感染的自然转归,有望成为临床上 HBV感染转归的预测指标。更多还原

【Abstract】 Objective To study the relationship between a G/T substitution at position -88 of myxovirus resisitence-1 gene (MxA) and the self-limiting or chronic infection of HBV. Methods Blood samples from 100 patients with self-limiting HBV infection (positive anti-HBs and anti-HBc) and from 340 patients with chronic HBV infection were collected. MxA-88 G/ T polymorphism was typed using a protocol based on competitively differentiated-polymerase chain reaction. For statistical analysis, odds ratio and x2 test were used. Results The detective rate of G/G genotype (low expression genotype) of MxA-88 G/ T was 50.2% (221/440), those of T/T genotype (high expression genotype) and G/T heterozygous genotype were 5.5% (24/440) and 44.3% (195/440). Compared to patients with chronic infection, patients with self-limiting infection had lower frequency of G/G genotype (41.0% vs 52.9%, P < 0.05) or G allele (62.5% vs 75.9%, P < 0.01) and had higher frequency of T/T genotype (16.0% vs 2.4%, P < 0.01) or T allele (37.5% vs 24.1%, P < 0.01), but there was no significant difference in the G/T heterozygous genotype. Conclusions MxA gene -88 G/T polymorphism influences the natural outcomes of HBV infection to some extent. This SNP of MxA gene may be used as a clinical prognostic marker of HBV infection.更多还原