【摘要】 目的探讨乳腺癌组织中血管内皮生长因子(VEGF)-(A、C、D)mRNA表达在乳腺癌发生发展中的作用及其与预后的关系。方法用TaqMan real-time逆转录聚合酶链反应检测61例乳腺癌和29例乳腺良性病变中VEGF-(A、C、D)mRNA的表达,分析其表达与乳腺癌临床病理参数及生存状况间的关系。结果(1)乳腺癌中VEGF-(A、C)mRNA表达量(2．79±1．31、3．33±0．88)高于良性乳腺病变(1．59±1．35、2．76±0．55),差异有统计学意义(P=0．000,P=0．002)。乳腺癌中VEGF-D mRNA表达阳性率(73．77％)高于良性乳腺组织(51．72％,P=0．038),但表达量二者间无差异,P=0．683。(2)单因素和多因素分析均显示VEGF-D与VEGF-C mRNA比值在淋巴结转移阳性组低于阴性组,且与淋巴结转移有关(P单=0．046,P多=0．062)。(3)乳腺癌VEGF-(A、C)mRNA高表达患者无病生存率分别低于低表达组(P=0．030,P=0．044)。结论VEGF-(A、C、D)mRNA表达异常可能与乳腺癌发生发展有关。VEGF—D与VEGF-C mRNA表达比值改变可能与乳腺癌淋巴结转移有关,VEGF-(A、C)是乳腺癌预后重要的影响因子。更多还原

【Abstract】 Objective To study mRNA expression of VEGF-( A,C,D) genes in breast carcinoma and its effect on prognosis. Methods The mRNA expression of VEGF-(A,C,D) genes in 61 cases of primary breast carcinoma and 29 cases of benign mammary lesions was detected by TaqMan real-time reverse transcription-polymerase chain reaction ( real-time RT-PCR) technology. The correlation between mRNA expression and various clinicopathologic parameters and survival data was analyzed. Results The level of mRNA expression of VEGF-(A,C) genes was significantly higher in primary breast carcinomas (2.79±1. 31 and 3. 33±0. 88 respectively) than in benign mammary lesions ( 1. 59±1. 35 and 2. 76±0. 55 respectively, P = 0. 000 and 0. 002 respectively ) . The percentage of VEGF-D mRNA expression was 73.77% in breast carcinomas, as compared to 51.72% in benign mammary lesions (P = 0.038). However, there was no statistically significant difference in the mRNA expression levels of VEGF-D gene between primary breast carcinomas and benign mammary lesions ( P = 0. 683 ) . On the other hand, the ratio of VEGF-D mRNA expression to VEGF-C mRNA expression was lower in lymph node-positive than in lymph node-negative cases. This also correlated with the occurrence of nodal metastasis by uni- and multivariate analysis (Puni =0. 046 and Pmult, =0. 062). High mRNA expression of VEGF-( A,C) genes was associated with poor disease-free survival ( P = 0. 030 and 0. 044 respectively). Conclusions The expression of VEGF-(A,C,D) genes may play a role in the disease progression of breast carcinoma. The ratio of VEGF-D mRNA expression to VEGF-C mRNA expression correlates with the occurrence of lymph node metastasis in breast carcinoma. The mRNA expression of VEGF-( A,C) genes may serve as a useful prognostic indicator in breast cancer.更多还原