【摘要】 参考GenBank中发表的猪瘟病毒(CSFV)序列,设计一对CSFV特异性PCR引物；从CSFV感染猪盐渍小肠中提取总RNA,经逆转录后进行PCR扩增,在盐渍小肠中成功扩增出与预期大小(168bp)一致的特异性条带,而正常猪和感染猪伪狂犬病病毒的猪小肠扩增结果均为阴性。用本方法对20例不同稀释浓度的盐渍猪肠衣样本进行检测,结果显示比经典抗原检测方法(抗原捕获ELISA法)具有更高的敏感性。实验表明,本RT-PCR技术能应用于盐渍猪肠衣的CSFV检测,为快速、准确检测盐渍猪肠衣中CSFV提供了一条新途径。更多还原

【Abstract】 A pair of specific primers was synthesized according to the sequences of classical swine fever virus(CSFV)pub- lished in GenBank.The specific expected fragment(168 bp)was amplified by RT-PCR from the total RNA extracted from salted casings of pigs infected CSFV whereas no amplification were detected in normal or pseudorabies virus(PRV)infected.Twenty salted swine casing samples were detected by this method,which showed the same results as antigen capture ELISA test.This method can be used for the fast and accurate detection of CSFV in salted swine casings.更多还原