【摘要】 目的了解福建省1996~2002年急性弛缓性麻痹(AFP)病例中检出病毒的型别分布及特点。方法用RD、HEp-2、L20B细胞从AFP病例粪便标本中分离病毒,阳性分离株首先应用脊髓灰质炎(脊灰)病毒(PV)3个型血清鉴定PV型别,PV3个型血清无法中和的病毒再用世界卫生组织(WHO)提供的RIVM诊断组合血清进行非脊灰肠道病毒(NPEV)型别鉴定,继而应用逆转录-聚合酶链反应(RT-PCR)及序列测定与分析对仍无法定型的病毒株进行鉴定,最后对其中鉴定为非EV疑似腺病毒的标本及2株新血清型EV应用电子显微镜技术从形态学方面进行诊断。结果1996~2002年AFP病例中139例病毒分离阳性,其中46例检出PV疫苗相关株,占33.09%,以Ⅱ型为主,其余93例在最初鉴定为NPEV。在能重新扩增出病毒且仍鉴定为NPEV的57例阳性病例标本中,除5例经电镜形态学诊断为腺病毒外,其余52例均为NPEV,涵盖了25个血清型,并以埃柯病毒(ECV)为主(77.78%),常见的有ECV3、11~13型,柯萨奇A、B,各占11.11%、7.41%,同时发现2例新血清型NPEV。结论与无脊灰野病毒前相比,1996~2002年分离的病毒已从1992~1994年以PV为绝大多数转变为以NPEV为主,分离的PV从以Ⅰ型脊灰野病毒为主转变以PVⅡ型疫苗相关株为主。NPEV中血清型分布广泛,不同血清型的流行规律及特点尚待进一步研究。更多还原

【Abstract】 Objective To learn serotype distribution of the virus isolates from acute flaccid paralysis patients(AFP) in Fujian Province during 1996-2002.Methods Viruses were isolated by cell culture with RD,Hep-2 and L20B cell lines.Firstly,these isolates were serotyped by the standard method of neutralization using the three types PV antisera,if the results were negative,secondly,they were identified using the pools of RIVM,and then were typed by RT-PCR and genomic sequencing.Lastly 5 adenovirus isolates and the two untyped enteroviruses were characterized by electron microscopy.Results Viruses were isolated from 139 cases during 1996-2002,including 46 Sabin-like polioviruses(33.09%),which predominant by PVⅡ and 93 non-polio viruses.Of the 57 positive cases redetected as non-polio viruses in this study,5 were adenovirus,52 were NPEV.Of the NPEV isolates,25 serotypes were represented.Echoviruses accounted for 77.78%,followed by coxsackievirus A(11.11%) and B(7.41%),and two novel serotypes have been detected.The four most common serotypes were echovirus 3,11,12,and 13.Conclusion Compared with 1992-1994 before polio-free,the predominant serotypes in 1996-2002 had converted from PV to NPEV,and among the PV isolates,the most common type had changed from wild PVⅠ to Sabin-like PVⅡ.The serotypes of NPEV in AFP cases distribute wildly.The rules and characteristics of the different serotypes prevalence should be studied.更多还原