【摘要】 目的研究几种维生素对肝纤维化大鼠的影响并比较其疗效。方法用维生素A(VA),β-胡萝卜素(β-CAR),维生素C(VC)和维生素E(VE)干预免疫性肝纤维化大鼠,通过肝组织病理学、肝纤4项(透明质酸、层粘蛋白、Ⅲ型前胶原和Ⅳ型胶原)、转化生长因子β1(TGFβ1)mRNA和金属蛋白酶组织抑制因子-1(tissue inhibitor of met-alloproteinase,TIMP-1)mRNA在肝组织中的表达等指标判定并比较其疗效。结果VA、β-CAR、VC和VE组的肝纤维化半定量计分分别为8.00±4.14,6.25±4.47,13.13±7.54,5.06±3.60,除VC外,其他3种维生素均对肝纤维化大鼠肝组织的病理变化有明显的改善作用;VA、β-CAR和VE组的血清肝纤4项综合得分均明显低于模型组,且VE组得分低于VA、与β-CAR组差异无统计学意义;VA,β-CAR和VE均能明显抑制肝纤维化大鼠肝组织TGFβ1mRNA的表达,VE还能明显抑制TIMP-1 mRNA的表达。结论VA、β-CAR和VE均有抗肝纤维化作用,且VE的效果优于VA,与β-CAR差异无统计学意义,VC无效。更多还原

【Abstract】 Objective To research and to compare the effects of several vitamin on rats with hepatic fibrosis. Methods The rats with immune hepatic fibrosis were treated by VA,β-CAR,VC and VE.The anti-fibrosis effects were evaluated and compared with histopathology of liver tissue,four fibrosis serum marker and the expression of TGFβ₁ mRNA and TIMR-1 mRNA in liver tissue. Results The semiquantitative scoring system（SSS）of liver tissue were 8.00±4.14,6.25±4.47,13.13±7.54 and 5.06±3.06 in VA,β-CAR, VC and VE group respectively.The SSS was significantly lower in VA,β-CAR and VE group compared with model group,but no significantly difference between VC and model group was found.The general scoring of four fibrosis serum markers in VA,β-CAR and VE group was lower than that in model group.The general scoring in VE group was also lower than that in VA and VC group but no significantly difference berween VE and β-carotene group.The expression of TGFβ₁?mRNA in liver tissue was markekly β-carotene group.The expression of TGFβ₁?mRMA in liver tissue was markedly inhibited by VA,β-CAR and VE and the expression of TIMP-1 mRNA in liver tissue was also inhibited by VE. Conclusion VA,β-CAR and VE all have anti-fibrosis effects on rats and VE is more excellent than VA on anti-fibrosis effects but no difference compared with β-CAR.No effects are found in VC group.更多还原