【摘要】 目的为预防控制婴幼儿病毒性腹泻的流行提供病原学和流行病学依据。方法以河北省卢龙县医院和妇幼保健院因腹泻住院治疗的患儿为监测对象,采用流行病学调查方法对腹泻病患儿进行检测调查。结果1 236份粪便样本轮状病毒阳性率为46%;398份轮状病毒阴性样本中杯状病毒阳性率为31.6%;2种病毒腹泻感染率以12月龄的患儿所占比例最高,分别为39.3%和27.4%,其中轮状病毒9月龄患儿感染率为26.1%,6月龄为14.4%;而杯状病毒6,9,3月龄患儿感染率分别为25.6%,17.9%和16.2%。轮状病毒和杯状病毒感染发病月份基本一致,主要集中在当年12月至次年2月,分别占全年病例的86%和65%。轮状病毒毒株鉴定结果:G3型(45%)最常见,其次为G1型(35%),G2型(8%),G4型(3%),G9型(0.6%),混合感染(1%),未分型(8%);杯状病毒经DNA测序均属于诺瓦克病毒(NLV)GⅡ遗传组。流行株为NLVGⅡ-4,GⅡ-7和GⅡ-3。结论婴幼儿病毒性腹泻的病原主要是轮状病毒,其次是杯状病毒。更多还原

【Abstract】 Objective To investigate the epidemiological characterstus of rotanvirus and caliciviruses among children under 5 years of age with acute diarrhea. Methods Two viruses were detected by enzyme linked immunosorbent assay and reverse transcription-polymerase chain reaction（RT-PCR）,some caliciviruses were cloned and sequenced. Results 1?236 fecal specimens were detected for rotavirus,46% was positive and 393 rotavirus negative which fecal specimens were detected for caliciviruses 31.6% was positive.The distribution of month-old The highest was 12 month group for rate of Two virus were infected.followed rotavirus was 9 month group（26.1%） 6 month group（14.4%） and calicivirus was 6 month group（25.6%）,9～month group（17.9%）.The distribution of seasons two riruses maily were infected in 12 month,1month and 2 month.Rotavirus Gserotyping results identified G₃（45%） to be the most common strain.followed by G₁（35%）,G₂（8%）,G₄（3%）,G₉（0.6%）.mixed infections（1%）and 8% of the strains remained untypeable.Caliciviruses.results identified:All strains belonged to NLVGVⅡ in which 6strains GⅡ-3,2 strains GⅡ-4 and 3 stains GⅡ-7. Conclusion Rotavirus is the main cause of diarrhea in chidren and caliciviruses is the next one.更多还原