【摘要】 目的研究膳食脂类对大鼠动脉窖蛋白-1(caveolin-1)表达的影响。方法雄性SD大鼠试验组用高脂饲料喂养15周诱导肥胖(DIO)和肥胖抵抗(DIO-R)后,将DIO组一半改用基础饲料喂养(DIO-HF/LF),另一半继续高脂饮食(DIO-HF);对照组(CF)一直用基础饲料喂养。第23周末用RT-PCR、免疫印迹及免疫组化方法检测动脉血管caveolin-1表达水平。结果DIO-HF/LF组体重低于DIO-HF组而高于对照组(P<0.05)。血管caveolin-1水平显示,DIO-HF高于DIO-HF/LF、DIO-R及CF,而其余3组之间差异无统计学意义。结论基础饮食可降低肥胖敏感大鼠体重及血管caveolin-1表达,降低心血管疾病的危险性。肥胖抵抗可能与caveolin-1维持正常表达有关。更多还原

【Abstract】 Objective To study the effect of dietary compositions on the expression of caveolin-1 in artery of rats.Methods Dietary induced obesity(DIO)and dietary induced obesity resistant(DIO-R)rats were induced by high-fat diet for 15 weeks.And half of the DIO rats were switched to standard diet(DIO-HF/LF),the other DIO rats(DIO-HF)and DIO-R rats continued their primary diet.Control group was fed with standard diet all through.At the end of the experiment by the 23rd week the rats were sacrificed and arteries were removed for measuring the expression of caveolin-1 by reverse transcription polymerase chain reaction(RT-PCR),western blotting and immunohistochemistry.Results The final weights of DIO-HF/LF were lower than that of DIO-HF rats whereas higher than that of CF rats.The level of caveolin1 expression in DIO-HF group was the highest among the four groups,and no significant difference was found among the other three groups.Conclusion Switched to standard diet of DIO rats could reduce the body weight gain and decrease the expression of caveolin-1 from high to the normal level,which might lower the risk of cardiovascular disease.DIO-R rats can remain normal expression of caveolin-1,which might be related to obesity resistance.更多还原