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早熟禾品种耐寒变异植株无性系的建立
The Clone Establishment of Cold Resistant Variation Plant of Poa pratensis Cultivars
【摘要】 对草地早熟禾的小矮人品种耐寒变异植株无性系的建立进行了研究,并成功地诱导出小矮人嫩叶的愈伤组织,分化出丛生不定芽。诱导愈伤组织的理想培养基是MS+BA 0.1mg/L+2,4-D 1 mg/L;由愈伤组织分化出苗的理想培养基是1/2 MS+BA 0.1 mg/L+2,4-D 0.1 mg/L;试管苗极易移栽成活,耐寒变异性状保持不变。
【Abstract】 The clone of cold resistant lawn plant-Poa pretensis was successfully established by inducing the callus and developing uncertain buds from its leaf.The best medium for callus induction was MS+BA 0.1mg/L+2,4-D 1 mg/L;medium consisting of 1/2 MS+BA 0.1 mg/L +2,4-D 0.1 mg/L was the best differentiation one for buds.Plantlets in tube were of the cold resistant character and with best rooting effect.
【关键词】 草坪植物;
小矮人;
愈伤组织;
组织培养;
无性系;
【Key words】 Lawn plant; Poa pratensis; Callus; Culture of tissue; Clone;
【Key words】 Lawn plant; Poa pratensis; Callus; Culture of tissue; Clone;
【基金】 辽宁师范大学教学改革项目(20050101123)
- 【文献出处】 中国草地学报 ,Chinese Journal of Grassland , 编辑部邮箱 ,2006年04期
- 【分类号】S688.4
- 【下载频次】51