【摘要】 目的:探讨附睾精子抽吸术(ESA)结合卵细胞内单精子注射(ICSI)技术治疗阻塞性无精子症所致男性不育的治疗效果。方法:选择2002年1月~2003年12月到我院治疗不育症确诊为阻塞性无精子症男性不育患者,采用ESA方法吸取男性附睾液,分离精子用于ICSI;同时按常规体外受精-胚胎移植方法(IVF-ET),采用GnRH-α+FSH/hMG+hCG促排卵方案对女性进行促排成熟卵细胞(M II)用于显微注射授精,受精卵体外培养3 d后移植回子宫内。结果:采用MESA结合ICSI技术治疗32周期阻塞性无精子症所致不育的夫妇,所获成熟卵(M II)162个,受精率66.28%,卵裂率62.21%,临床妊娠率31.20%。结论:采用ESA结合ICSI技术治疗阻塞性无精子症所致男性不育获得良好的效果,该方法为阻塞性无精子症男性不育患者提供了一种快速、方便、无痛、有效的治疗方法。更多还原

【Abstract】 Objective:To investigate the efficacy of intracytoplasmic sperm injection(ICSI) combined with epididymal sperm aspiration(ESA) in the treatment of obstructive azoospermia induced male infertility.Methods:From January 2002 to December 2003,12 cycles were selected,ESA were adopted to aspirate epididymal sperms for ICSI,and ovarian stimulation was achieved on routine in vitro fertilization-embryo transfer(IVF-ET) protocols.All metaphase Ⅱ(MⅡ) oocytes were selected for micro-sperm injection,the embryos were transferred back to the uterine 3 days after insemination.Results:62 MⅡoocytes were injected in 12 cycles,the insemination rate and embryo cleavage rate was 66.28% and 62.21% respectively,two couple pregnancy and clinic pregnant rate was 20%.Conclusion:ESA combined with ICSI is an effective approach for the treatment of obstructive azoospermia induced male infertility and also a rapid,convenient method for clinic male infertility patients.更多还原