【摘要】 目的观察REDD1mRNA在癫痫患者脑组织中的表达及临床意义。方法将50例癫痫患者分成难治性(42例)和非难治性癫痫(8例)两组,用基因芯片和免疫荧光定量PCR分别检测脑组织中REDD1mRNA基因表达,并与对照组进行比较。结果基因芯片扫描提示REDD1mRNA在癫痫患者脑组织中与对照组比较表达明显增加,难治性癫痫组和非难治性癫痫组没有明显的表达差异,免疫荧光定量PCR的结果与基因芯片结果一致。结论 REDD1mRNA在癫痫患者脑部中存在高表达,这种高表达可能通过雷帕霉素哺乳动物靶标系统导致神经元坏死与凋亡。更多还原

【Abstract】 Objective To investigate the expression and clinical signification of REDD1 (regulated in development and DNA damage responses, REDD1) mRNA in the brain tissue of the epilepsy patients. Methods Intractable epilepsy(IE) and non-intractable epilepsy(NIE) were studied by using gene microarray screening and real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) for REDD1 mRNA. Results In this study, the cDNA microarray analysis showed that REDD1 mRNA level in epilepsy patients was significantly higher than that of the control group. However,there was no difference between IE patients and NIE patients. The results of FQ-PCR were coincided well with the cDNA microarray datas. Conclusion Our study suggests that upregulation of REDD1mRNA is seen in the patients with epilepsy. The gene expression of REDD1 in epilepsy is significantly higher than those of control brain samples. It might lead to the neural damage or apoptosis through the mammalian target of rapamycin.更多还原