【摘要】 目的:了解SARS-CoV抗原免疫鸡后,卵黄抗体效价的变化。方法:卵黄1∶1 600倍稀释后ELISA方法测定抗体效价。结果:Cutoff为0.21±0.05,在首次免疫26-28 d后,抗体效价达到最高(1∶1 600稀释(A450=1.3),并且抗体持续高效价(A450>0.8)。随着免疫时间的延长,抗体效价降低;间隔一定时间加强免疫,抗体效价又回升达到高峰。结论:SARS-CoV抗原持续免疫鸡,卵黄抗体持续保持高效价。更多还原

【Abstract】 AIM: To observe the change of yolk antibody titers after immunizing hens with SARS-CoV antigen.METHODS: Yolk antibody diluted with water to（1∶1 600） was determined with ELISA methods.RESULTS: The Cutoff is 0.21±0.05.After immunization,antibody titers in hens reached the peak in first 26-28 day(A₄₅₀=1.3 when antibody was diluted as（1∶1 600）),and hold high titers(A₄₅₀>0.8).The antibody titers decreased after the time of immunity.The increase and peaking of antibody titers were observed at intervals when repeated immunizations were given.CONCLUSION: Yolk antibody kept high titers when hens were immunized with SARS-CoV antigen.更多还原