【摘要】 目的:探讨肝硬化及肝纤维化对尿苷二磷酸葡萄糖醛酸基转移酶(UGT)表达水平的影响。方法:应用RT-PCR和Southern杂交技术,观察肝硬化患者肝脏组织中UGT同工酶mRNA表达水平的差异,及肝纤维化对UGT表达水平的影响。结果:肝硬化组UGT2B15、UGT1A6的mRNA水平高于对照组分别为227%和166%;肝纤维化对UGT2B15RNA的表达有显著的影响,4级肝纤维化患者UGT2B15mRNA水平为0级肝纤维化的172%、1级的208%、2级的243%。3级肝纤维化患者UGT2B7mRNA水平为0级肝纤维化的192%、1级的208%、2级的156%。结论:肝硬化可影响肝脏葡萄糖醛酸转移酶同工酶的表达水平;肝纤维化对UGT mRNA的表达有显著的影响。更多还原

【Abstract】 AIM: To investigate whether cirrhosis affects the expression of hepatic glucuronosyltransferase (UGT) isoforms and its clinical significance. METHODS: Reverse transcription and polymerase chain reaction (RT-PCR) and Southern hybridization were used to determine the mRNA levels of five UGT isoforms in hepatic tissues. RESULTS: Compared to control group, UGT2B15 and UGT1A6 mRNA level in cirrhosis group increased by 227% and 166%, respectively. Compared to 0 fibrotic grade group, UGT2B15 mRNA levels of 1, 2, 4 fibrotic grade patients were 172%, 208% and 243%, respectively. UGT2B7 mRNA levels of 1, 2, 3 fibrotic grade patients were 156%, 208% and 192%, respectively. Inflammation grade showed no obvious effect on mRNA expression of most UGT isoforms. CONCLUSION: Cirrhosis affectes individual UGT isoform mRNA level. Degree of fibrosis has a significant effect on UGT mRNA expression.更多还原