【摘要】 对来自国内不同地区、不同蔬菜作物上的8个黄瓜花叶病毒分离物,采用一步法RT-PCR扩增得到CP基因全长片段,分别克隆到pMD-18T载体并测序。结果表明,本研究8个分离物的CP基因均为657个碱基,可编码218个氨基酸,它们之间的核苷酸序列同源性较高,达92·8%~99·1%;将它们与GenBank中部分CMVCP基因序列比较,显示出与CMV亚组Ⅰ株系的核苷酸序列同源性达90·7%~98·0%,而与亚组Ⅱ株系的核苷酸序列同源性仅有75·8%~78·1%。因此,这8个分离物应归属于CMV亚组Ⅰ。更多还原

【Abstract】 The eight CP genes of CMV isolated from different areas and vegetables in China were amplified by one-step RT-PCR, and transformed into pMD-18T vector and then sequenced, respectively. The results showed that the sequences of the eight CP genes were all 657 nucleotides that could encode the protein of 218 amino acids of each isolate, and the isolates shared high sequence homology from 92.8% to 99.1% among them. The nucleotide sequence alignment showed, compared with some CP gene sequences of CMV reported in GenBank, the eight CP genes shared 90.7%-98.0% homology with those of CMV subgroup I strains, whereas only shared 75.8%-78.1% homology with those of CMV subgroup Ⅱ strains. Therefore, it was supposed that all the eight CMV isolates reported in this study belonged to the subgroup I of CMV.更多还原