【摘要】 目的:建立紫杉醇聚乳酸钠米粒含量测定的方法。方法:采用反相高效液相色谱法,以ODS C18柱为固定相,甲醇-水(75:25)为流动相,检测波长为227 nm,流速为1．0 mL/min。结果:回归方程为A=0．648 798 C-0．027 076,r=0．999 9;总的紫杉醇浓度及游离紫杉醇浓度的测定方法的平均回收率分别为(100．42±3．99)％(n=9)和(100．51±2．01)％(n=9);日内精密度RSD≤1．75％,日间精密度 RSD≤1．23％;纳米粒胶体中药物含量为(34．83±2．64)μg/mL,包封率为(99．30±0．10)％。结论:该方法可用于紫杉醇聚乳酸纳米粒的含量测定。更多还原

【Abstract】 Objective: To determine paclitaxel(TAX) in paclitaxei - loaded PLA nanoparticles. Methods: An RP-HPLC was developed, the paclitaxel was separated on octadecylsilane column using a mobile phase of methanol water(75 : 25) with detection at 227 nm, the flow rate was 1.0 mL/min. Results: The standard curve was A =0. 648 798C-0. 027 076, r=0. 9999, the average recovery rate of the total drug and the free drug in the nanoparticle colloid solution were (100. 42±3.99)% ( n =9) and (100. 51±2. 01)% ( n =9) respectively. And the precision within day was less than or equal to 1.75%, the precision between day was less than or equal to 1.23%. And the paclitaxel concentration in the nanoparticle colloid solution was (34. 83±2. 64)μg/mL, the extent of entrapment of the nanoparticles was (99. 30±0.10)%. Conclusion: The method could be used to quantitate paclotaxel.更多还原