【摘要】 目的:建立环维黄杨星 D 的含量测定方法。方法:采用 Apollo C₁₈柱（4.6 mm×250 mm,5μm）,流动相为5 mmol·L^-1庚烷磺酸钠溶液（内含0.15%的三乙胺,磷酸调节 pH 至3.1）-乙腈（73:27,v/v）,流速1.0 mL·min^-1,检测波长203 nm。结果:环维黄杨星 D 主峰峰形对称,与杂质峰分离良好。在0.12～0.72 mg·mL^-1范围内线性关系良好（r=0.9994）,平均回收率（n=3）为98.4%～99.0%,RSD 为0.3%～0.9%。结论:本法操作简单、结果准确,具有良好的重复性,可用于环维黄杨星D的含量测定。更多还原

【Abstract】 To establish a method using IP-HPLC for the determination of eyclovirobuxium D.Methods: The assay was conducted on a Apollo C₁₈column（4.6 mm×250 mm,5μm）with 5 mmol·L^-1solution of sodium heptanesulfonate（mixed 0.15% triethylamine and adjusted to pH 3.10 with H₃PO₄）-acetonitrile（73:27,v/v）as mobile phase.Flow rate was 1.0 mL·min^-1.The detection wavelength was set at 203 nm.Results:Cyclovirobuxi- um D obtained good isolation from other substances.The calibration curve was linear in the range of 0.12- 0.72 mg·nof cyclovirobuxium D with correlation coefficient 0.9994.The average recovery（n=3）of cyclov- irobuxium D was 98.4%-99.0%,RSD was 0.3%-0.9%.Conclusion:The method is simple,rapid,and ean be used for the determination and quality control of cyclovirobuxium D with satisfactory accuracy and reproducibility.更多还原