【摘要】 以小桐子(Jatrophacurcas)的胚芽、子叶、下胚轴、叶柄、叶片和茎段作为外植体,用不同浓度的6-苄基腺嘌呤(6-BA)和α-萘乙酸(NAA)对其进行愈伤组织的诱导和植株再生的研究。结果表明在MS培养基中加入5.0mg/L6-BA和1.0mg/LNAA对愈伤组织的诱导效果最好;加入5.0mg/L6-BA和0.1mg/LNAA对不定芽的诱导最为有效,加入0.1mg/L6-BA和1.0mg/LNAA有利于芽的生长;加入1.0mg/LNAA的1/2MS培养基对生根最为有利。更多还原

【Abstract】 In this paper,plumules,cotyledons,hypocotyls,leaf blades,petioles and stalks of physic nut (Jatropha curcas L.) were used as explants,and callus induction and plant regeneration were studied on MS medium contained different concentrations of 6-BA and NAA.The results showed that the MS medium with 5.0?mg/L BA and 1.0?mg/L NAA was the best for callus induction,and with 5.0?mg/L BA and 0.1?mg/L NAA,for formation of adventitious buds,and with 1.0?mg/L BA and 1.0?mg/L NAA,for bud growth,and that 1/2 MS medium with 1.0?mg/L NAA was the best for formation and growth of adventitious roots.更多还原