【摘要】 对大肠杆菌、枯草杆菌、四联球菌、普通变形杆菌、产气肠杆菌基因组DNA进行rep-PCR扩增,并在模板样品中混入植物DNA作对照实验.结果表明:用rep-PCR方法分析细菌基因多样性,能在较大范围内避免植物基因的干扰.模板量梯度实验结果显示:扩增产物多态性与模板量相关.更多还原

【Abstract】 The genomic DNAs of Escherichia coli,Bacillus subtilis,Micrococcus tetragenus,Proteus vulgaris and Enterobacter aerogenes were analyzed by rep-PCR with BOX primers,and the CK experiment was carried out by adding plant genomic DNAs into the bacteria genomic DNAs.It was showed that the repPCR with BOX primers could identify these bacteria and the bacteria polymorphism was not interfered by plant genomic DNAs in a large range of DNA template concentration.It was also showed by the results from the gradient experiment with template quantities that the bacteria rep-PCR polymorphism was related to template concentration.更多还原