【摘要】 目的探讨凋亡抑制基因Survivin在食管癌患者外周血中表达的临床意义。方法共93例食管良、恶性病变患者,采用荧光定量PCR法,检测外周血Survivin m-RNA,分别观察其在食管良性病变、原位癌及浸润癌患者外周血中的表达强度。结果Survivin m-RNA在食管良性病变、原位癌及浸润癌患者外周血中均呈渐进性高表达,并与病期有关。结论外周血中Survivin m-RNA表达强度与食管癌的发生、发展密切相关,实时荧光定量PCR法检测食管癌外周血中Survivin m-RNA,可方便食管癌的诊断及指导治疗。更多还原

【Abstract】 Objective To investigate the relationship of apoptotic gene Survivin in the peripheral blood of esophageal cancer patients.Methods Real-time fluorescence quantitative PCR was used to detect the expression of Survivin m-RNA in the peripheral blood of 93 patients with different esophageal lesions,including benign lesion,canser in situ,and invasive carcinoma.Results The expression of Survivin m-RNA in the peripheral blood of these patients increased progressively from benign lesion to invasive carcinoma,and was related with staging.Conclusion The expression of Survivin m-RNA in the peripheral blood is closely related with the genesis and progression of esophageal carcinoma.Real-time fluorescence quantitative PCR used to detect the expression of Survivin m-RNA in the peripheral blood may be more convenient for the diagnosis and guidance of treating esophageal carcinoma.更多还原