【摘要】 目的考察淫羊藿与黄芪按不同比例配伍时淫羊藿主要成分的变化。方法将淫羊藿与黄芪分别按1∶0、2∶1、1∶1、1∶2、0∶1比例配伍,水煎,制备供试样品,以RP-HPLC法测定淫羊藿苷的含量,比较优化的HPLC色谱条件下的指纹图谱。结果淫羊藿与黄芪配伍后色谱峰具有加合性,未发现新峰产生。淫羊藿中淫羊藿苷等多种成分的含量不同程度的增加,其中以1∶1比例配伍时含量增加最明显,有些成分含量则无明显改变。结论淫羊藿与黄芪配伍煎煮能促进淫羊藿中多种成分的煎出,配伍比例为1∶1时该作用更明显。这为二药配伍增效作用研究提供了一定的参考。更多还原

【Abstract】 Objective To explore the variation of the main chemical constituents of Herb Epimedii in decocting Herb Epimedii and Radix Astragali separately and compatibly. Methods Herb Epimedii combined with Radix Astragali were decocted with water in which the proportion of them were 1∶0, 2∶1, 1∶1, 1∶2 and 0∶1, respectively. RP-HPLC was used to compare the content of icariin and the fingerprints in separate and compatible decoction. Results Afeter combination, the number of chromatographic peaks were added and no new peaks were detected. The contents of some chemical constituents such as icariin in Herb Epimedii were increased, especially in the compatible decoction at the proportion was 1∶1. Some constituents were not changed obviously. Conclusion Herb Epimedii decocted together with Radix Astragali especially at a ratio of 1∶1 can increase the solubility of the chemical constituents in Herb Epimedii. This provides a scientific basis for more effective use of the compatible decoction of the two herbs.更多还原