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稀土杂多配合物K15[Ce(BW11O39)2]·17H2O与BSA的相互作用

Study on the Interaction between Rare Earth Salts of Heteropoly K15[Ce(BW11O39)2]·17H2O and Bovine Serum Albumin

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【作者】 曾芳杨昌英潘家荣赵儒铭周百斌

【Author】 ZENG Fang~1,YANG Chang-ying~1,PAN Jia-rong~1,ZHAO Ru-ming~(2),ZHOU Bai-bin~3(1.School of Chemistry and Life Science,Three Gorges University,Yichang 443000,China;2.Hubei Three Gorges Vocational and Technical College,Yichang 443002,China;3.School of Chemistry and Physics,Harbin Normal University,Harbin 150080,China)

【机构】 三峡大学化学与生命科学学院湖北三峡职业技术学院哈尔滨师范大学理化学院 湖北宜昌443000湖北宜昌443000湖北宜昌443002黑龙江哈尔滨150080

【摘要】 利用紫外光谱,以曙红Y(Eos in Y)为探针,考察了稀土杂多配合物K15[C e(BW11O39)2].17H2O与牛血清白蛋白(BSA)间的作用,K15[C e(BW11O39)2].17H2O与Eos in Y在BSA上有竞争结合,表面活性剂对两者间的竞争结合有增敏作用;并利用荧光光谱直接考察了K15[C e(BW11O39)2].17H2O与BSA的相互作用及其对BSA构象的影响。

【Abstract】 In this paper,the interaction between the rare earth salts of heteropoly acid,K15[Ce(BW11O39)2]·17H2O and bovine serum albumin(BSA) was studiedwith absorptionandfluorescence spectroscopy.In absorption spectroscopy,Eosin Y,as a protein probe,had bound to BSA,and the addition of heteropoly acid partial exchange with the Eosin Y,which is an indication that heteropoly acid can easily bind with BSA.Moreover,we demonstrated that the fluorescence quenching of BSA by Ce(BW11)2is a result of the formation of BSA-Ce(BW11)2complex.The effect of Ce(BW11)2on the conformation of BSA was analyzed using synchronous fluorescence spectroscopy.

【基金】 湖北省自然科学基金(SDI200232);宜昌市科技攻关项目资助(A04401-3,A032081)
  • 【文献出处】 稀土 ,Chinese Rare Earths , 编辑部邮箱 ,2006年04期
  • 【分类号】O641.4
  • 【被引频次】4
  • 【下载频次】136
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