【摘要】 建立了用高效液相色谱法测定茶叶中噻嗪酮农药残留量的方法,样品用乙腈提取,弗罗里硅土柱净化,用乙腈-0.1%磷酸水溶液做流动相,紫外检测器245nm检测,外标法定量。添加浓度在0.05～2.0mg/kg时,平均回收率为84.5～95.4%,相对标准偏差<7.60%,噻嗪酮的最小检出量为0.03mg/kg。该方法的灵敏度、准确度和精密度均符合农药残留测定的技术要求。更多还原

【Abstract】 A method for the determination of buprofezin residue in tea was established by HPLC with UV detector.The tea sample is extracted by acetonitrile,cleaned up by Florial column,eluted by petroleum ether-acetone(8:2,v/v).The samples were separated on TC-C18 column,with acetonitrile-water(60:40,v/v) as mobile phase.The samples were detected at 245nm with UV detector.The average recovery of method varies from 84.5 to 95.4% and the relative standard deviation is less than 7.60% when the sample was spiked at 0.05～2.0mg/kg.The lowest detection limit for buprofezin was 0.03mg/kg.The proposed method is characterized by simplicity,higher sensitivity and accuracy.更多还原