【摘要】 目的:探讨钙蛋白酶10基因多态性与原发性高血压遗传易感性的关系。方法:采用病例对照研究设计,以聚合酶链式反应-等位基因特异性扩增法(PCR-ASA),检测Calpain10基因SNP43G/A位点和SNP44T/C位点基因型,并进行与原发性高血压的关联分析。结果:在非糖尿病人群中,原发高血压患者和血压正常者的钙蛋白酶10基因SNP43GG基因型频率分别为70.59%和72.73%,差异无统计学意义,其SNP44TT基因型频率分别为80.88%和78.41%,差异亦无统计学意义;分析原发性高血压与血糖、真胰岛素、C肽及胰岛素抵抗指数HOMA-IR等的关系,高血压患者与正常人群各项临床指标之间的差异无统计学意义(P>0.05)。结论:钙蛋白酶10基因与昆明高原地区人群原发性高血压的发病无关,同时亦未发现原发性高血压与胰岛素抵抗的相关性。更多还原

【Abstract】 Objective:To explore the hypothesis that Calpain10 gene contributes to the genetic susceptibility in hypertension in plateau population.Methods:In case control study,PCR-ASA method was used to determine the distribution of allele and genotype frequencies of SNP43 polymorphism(G/A)and SNP44 polymorphism(T/C)of calpain10 gene in 68 hypertensive patients and 88 normal control subjects.Results:The frequency of "GG"genotype of the SNP43 in hypertensive patients is as same as that in the control subjects;and so is the frequency of "TT"genotype of the SNP44.The variation of clinical metabolic parameter in hypertensive patients is also as same as that in the control subjects.Conclusion:The study suggested that Calpain10 gene is not associated with hypertension in plateau population in Kunming;and the correlation between hypertension and insulin resistance is not found either.更多还原