【摘要】 目的研制复方甘草和辛夷(甘辛)鼻用抗过敏喷雾剂。方法以甘草提取物甘草甜素和辛夷花提取液为主药制备成鼻用喷雾剂,建立RP-HPLC测定甘草酸含量,以2.4-二异氰酸甲苯酯(TD I)介导的豚鼠超敏反应为模型,观察甘辛喷雾剂模型对过敏性鼻炎的疗效。结果制剂制备工艺可行,质量稳定,RP-HPLC对甘草酸铵含量测定结果可靠,重现性好;甘辛喷雾剂大小两个剂量组均在一定程度上使鼻黏膜上皮下嗜酸性粒细胞、淋巴细胞、浆细胞和嗜中性粒细胞减少,腺体增生和分泌亢进得到有效的抑制。结论甘辛喷雾剂可有效抑制超敏反应过程,保护鼻黏膜组织结构。更多还原

【Abstract】 OBJECTIVE To prepare the anti-allergic rhinitis dose form of compound Glycyrrhizin and Flos Magnoliae(Ganxin) nasal spray.METHODS The nasal spray was prepared by using the main drugs of the licorice extraction such as Glycyrrhizin and the Flos Magnoliae extraction.The method of RP-HPLC was established to determine the contents of glycyrrhizic acid.Models of guinea pig’s allergic rhinitis mediated by toluene-2,4-di-iso-cyanate(TDI) were applied to observe the effects of this spray on the allergic rhinitis.RESULTS The technique of the preparation was feasible,and the quality was stable.the results of the contents of monoammonium glycyrrhizinate determined by RP-HPLC were reliable and repeatable.the Ganxin spray both in small and large doses,to some extent,reduced the number of eosinophilic granulocyte,neutrophilic granulocyte and lymphocyte,and effectively inhibited the gland hyperplasia and the hypersecretion.CONCLUSION Ganxin spray can successfully inhibit the process of the type I allergic reaction and protect the structure of nasal mucosa.更多还原