【摘要】 目的:观察分析脑淋巴引流阻断对脑形态和功能的影响。方法:实验于2003-01/08在泰山医学院脑微循环研究所完成。实验动物选择雄性健康成年Wistar大鼠64只。49只大鼠用于脑淋巴引流对脑形态学的影响实验,随机分成3组;大体观察组14只;光镜观察组21只和电镜观察组14只。每组又分为脑淋巴引流阻断亚组和假手术对照亚组。16只动物用于脑淋巴引流阻断对皮质诱发电位影响的观察,随机分成两组,假手术对照组6只,脑淋巴引流阻断组10只。脑淋巴引流阻断亚组通过摘除颈浅和颈深淋巴结造成脑淋巴引流障碍,假手术对照组不结扎淋巴管和摘除淋巴结,通过大体,光镜及电镜观察不同时间(淋巴引流阻断1,2,3,5,7,10,15d)脑结构变化以及皮质诱发电位潜伏期的改变。结果:64只大鼠在实验过程中无死亡,均进入结果分析,无脱失值。①在脑淋巴引流阻断大鼠,大体观察见脑表面苍白、饱满、脑沟变浅窄、脑回变宽平,光镜下组织间隙增宽,液体郁滞,神经元变性、坏死、并有大量吞噬细胞浸润,形成“卫星现象”,电镜下神经元肿胀,线粒体等亚细胞结构变化明显。②与假手术对照组比较,脑淋巴引流阻断后第5天至第7天皮质诱发电位潜伏期明显延长[(6.28±0.23),(6.97±0.348)ms,P<0.01];(6.23±0.22),(7.12±0.20)ms;P<0.01]。结论:脑淋巴引流阻断对脑形态结构有重要影响,脑淋巴引流的阻断引起脑内感觉冲动传导异常,皮质诱发电位潜伏期明显延长。皮质诱发电位检查简便易行,可作为淋巴引流障碍性脑损伤的一项重要指标。更多还原

【Abstract】 AIM: To investigate the effect of cerebral lymphatic block on form and function of the brain. METHODS: The experiment was completed at Institute of Cerebral Microcirculation of Taishan Medical College from January to August 2003. Totally 64 Wistar rats were used in the experiment. Forty-nine rats were used for the morphological observation and the animals were divided into three groups: naked eye observation group (n=14), light microscopic observation group (n=12) and electronic microscopic observation group (n=14). The rats in each group were divided into cerebral lymphatic block subgroup and sham operation subgroup. Sixteen rats were used for the observation of the changes of cortical evoked potential, in which the animals were divided into sham-operation group (n=6) and cerebral lymphatic block group (n=10). Cerebral lymphatic blocked rat models were established by removing cervical lymphatic nodes. Morphologic changes of the brain and alterations of latency of cortical evoked potential were detected on the 1st, 2nd, 3rd, 5th, 7th, and 10th days after operation under naked eye, light microscope and lectronic microscope. Cortical evoked potential was also determined after the blockage. RESULTS: Totally 64 rats entered the final analysis without any loss. ① The brain swell, cerebral gyrus wide and flattened sulci after cerebral lymphatic block were observed under naked eye; and cerebral tissue space prolongation, increased interstitial fluid, neuronal degeneration and necrosis, diffused phagocytes and satellitosis were observed under light microscope. Neoronal swell and necrosis, glial cell swell, apparent subcellular changes such as mitochondron were observed under electric microscope. ② As compared with sham-operation group, latency of cortical evoked potential in cerebral lymphatic blockage group prolonged on the 5th day and 7th day after cerebral lymphatic obstruction (6.28±0.23), (6.97±0.348) ms,P < 0.01 ; (6.23±0.22), (7.12±0.20) ms; P < 0.01 . CONCLUSION: Cerebral lymphatic block plays a key role in cerebral morphology, and may result in abnormality of sensitive impulse conduction and prolong latency of cortical evoked potential. Examination of cortical evoked potential is easy and convenient, so it is regarded as a key index for lymphatic disturbed cerebral injury.更多还原