【摘要】 合成了六氮杂镍的配合物(Ni(Ⅱ)L-1,8-二羟基乙基-1,3,6,8,10,13-六氮十四烷高氯酸镍).用电化学、荧光光谱及黏度等方法研究了配合物与小牛胸腺DNA(ct-DNA)的相互作用.电化学实验表明,Ni(Ⅱ)L在Tris-HCl缓冲溶液(pH=7.3)中的氧化还原是1电子转移过程,Ni(Ⅱ)和Ni(Ⅲ)配合物对DNA键合常数的比值(K2+/K3+)为3.2;荧光实验表明该配合物与DNA具有明显的嵌插作用,其结合常数为2.15×104L/mol;黏度实验表明Ni(Ⅱ)L可使DNA发生卷曲或扭结作用.实验均表明Ni(Ⅱ)L和DNA相互作用为部分嵌插和部分沟谷结合的混合模式.在H2O2存在下,凝胶电泳和高效液相色谱方法证明Ni(Ⅱ)L对ct-DNA和pBR322 DNA的劈裂能力均增强.更多还原

【Abstract】 The hexaaza macrocyclic nickel（Ⅱ） complex（Ni（Ⅱ）L-1,8-dihydrox yethyl-1,3,6,8,10,13-hexaaza cyclotetradecane nickle（Ⅱ） perchlorate monohydrate） is synthesized.The interaction of Ni（Ⅱ）L with calf thymus DNA is studied using electrochemical techniques,emission and viscometry measurements.The cyclic voltammetry（CV） has showed that the ratio of the DNA binding constants(K₂₊/K₃₊) of the Ni（Ⅱ） and Ni（Ⅲ） complexes is 3.2.DNA competition binding with ethidium bromide indicates that Ni（Ⅱ）L binds to DNA in an intercalative function with a Stern-Volmer quenching constant(K_sv) of 2.15×10⁴ L/mol.The viscosity measurements suggest a partial intercalative mode.In both the presence and the absence of H₂O₂,it is evident that the Ni（Ⅱ）L complex can effectively cleave DNA as evidenced by agarose gel electrophoresis and high performance liquid chromatography measurements.更多还原