【摘要】 采用活性跟踪法从紫花地丁的氯仿萃取物中分离得到1个抗菌活性成分(化合物1),并以停乳链球菌、金黄色葡萄球菌、大肠杆菌、无乳链球菌、乳房链球菌和沙门氏菌为供试菌,对紫花地丁的抑菌活性及其活性成分进行了初步研究。结果表明,化合物1初步鉴定为黄酮类化合物;紫花地丁的主要活性成分位于甲醇-水(V(甲醇)∶V(蒸馏水)=1∶1)萃取物和氯仿萃取物中;化合物1对以上6种供试菌的最小抑菌浓度依次为0.039,0.078,0.156,0.313,0.625和1.25mg/mL;对金黄色葡萄球菌、无乳链球菌和乳房链球菌的最小杀菌浓度≤2.5mg/mL,对停乳链球菌、沙门氏菌和大肠杆菌的最小杀菌浓度>2.5mg/mL。更多还原

【Abstract】 Antibacterial activity of Viola yedoensis Makino and its antibacterial constituents were studied in this paper against S.aureas,S.agalactiae,S.uberis,S.dysagalactiae,E.coli and Salmonella in vitro.One antibacterial constituent (compound 1) from the chloroform extract was obtained with tracing-antibacterial activity method.The results showed that compound 1 was preliminarily identified as flavone;that main antibacterial comstituents in V.yedoensis Makino existed in chloroform phase and ethanol-water (1∶1) phase,that minimal inhibitory concentrations (MIC) of compound 1 were 0.039,0.078,0.156,0.313, 0.625 and 1.25 mg/mL respectively against S.dysagalactiae,S.aureas,E.coli,S.agalactiae,S.uberis and Salmonella,and minimal bactericidal concentrations (MBC) were ≤2.5 mg/mL against S.aureas,S.agalactiae and S.uberis,and >2.5 mg/mL against S.dysagalactiae,E.coli and Salmonella.更多还原