【摘要】 目的研究以3-巯丙基三乙氧基硅烷作为Fe3O4磁性粒子表面修饰剂,合成微米级磁响应性好且巯基含量较高的功能化磁性微粒,并对磁性微粒粒径以及表面巯基含量进行表征。方法激光粒度散射仪对功能化磁性微粒进行粒径分析,傅立叶变换红外光谱(FTIR)对巯基修饰前、后磁性微粒表面的功能基团进行表征,E llman方法对功能化磁性微粒表面巯基含量进行定量测定。结果氮气保护,乙醇与水体积比1∶1作为溶剂,500μL浓氨水为催化剂,加入300μL硅烷化试剂与170 mg磁性Fe3O4粒子充分反应,合成了平均粒径约为5μm的巯基化磁性微粒,所建立E llman方法对功能化磁性微粒表面巯基含量的测定结果为357μmol/g。结论功能化磁性微粒表面巯基的修饰及定量测定,为其在生物及医学领域的应用奠定了基础。更多还原

【Abstract】 Aim To synthesize Fe₃O₄ magnetic particles terminated with-SH group in different condition,the ratio of the Fe₃O₄ magnetic particles to 3-mercaptopropyltriethoxysilane,the catalyst and the protection atmosphere.The magnetic particles modified with-SH have small size,good magnetically responsive property and high amount of thiols.Methods Malvein particle size analyzer was used to analyze the size distribution of functional Fe₃O₄ particle,the thiol-functional Fe₃O₄ magnetic particle was characterized by the FTIR spectrum and Ellman′s method was established and used for quantitatively analyzing the-SH group on particle surface respectively.Results Under the condition of N₂,500μL concentration ammonia as the catalyst,300 μL of 3-m ercaptopropyltriethoxysilane and 170 mg of Fe₃O₄ were added to mixture solvent（ethanol∶water = 1∶1） and reacted for 6 h,the functional magnetic particles with 5 μm average diameter was synthesized,the amount of thiol groups was 357 μmol/g determined by means of Ellman′s method.Conclusion The modification and the quantitative methods for-SH groups on magnetic particle surface provide potential application for biological and medical areas in the near future.更多还原